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. As shown in Fig
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. Protein thermal aggregation involves distinct regions: sequential events in the heat-induced unfolding and aggregation of hemoglobin
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. Although gSBA was seen to demonstrate very high conformational stability, rSBA exhibits comparatively much lower firmness to resist the structural perturbation induced by the denaturant guanidinium hydrochloride
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. ANCOVA also showed no significant difference in elevations (P=0
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. These rates are much faster than the overall rate constant for ribozyme docking observed in previous ensemble kinetic studies, which is on the order of 10^sup -2^ s^sup -1^ for a 2WJ ribozyme construct (N
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