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Furthermore, the K^sub sv^ values were higher than those measured at neutral pH but smaller than those at acidic pH and in 7 M GdmHCl (Table 1 )
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Details about site-directed mutagenesis, protein expression, isolation, and purification of mutants I7S, F110S, and C3A/ C26A have been described elsewhere (22,24)
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The onset of this transition at various scan rates is observed at T^sub R^ = 32-34°C, similar to T^sub R^ interred from the CD data
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85, corresponding to donor-acceptor distances of ~64 [Angstrom] and 40 [Angstrom], respectively
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67, respectively; again, the effect was significant, F(1, 11) = 2525
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