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The quaternary structure of GR in the presence of GdnHCl was also investigated by size-exclusion liquid chromatography
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However, those authors propose that this phenomenon is unlikely to have physiological relevance, since the inactivation of GR is very slow at cellular NADPH concentrations
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One of the best-documented cases of pleiotropy is the gene associated with sickle-cell disease
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8 b shows the distribution of the iwo types of interactions
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The other class, comprising all other α-amylases, exhibit tryptophan residues that are much more buried in the interior of the protein (see Fig
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A rough estimate of the minimum number of binding sites, as done above with azurin, yields n ~20, which is four times larger than the corresponding value in liquid solution (5)
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5 T/m is required
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A minimum estimate of the anisotropy of 1
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We envision the use of these probes and other probes with similar properties and the application of some of the techniques described within to serve as useful tools for future scientific studies, diagnosis, and high throughput screening of misfolding inhibitors
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The fluorescence emission peak has here shifted to 482 nm, which was not noted in the case of increased viscosity
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, 1999), but which do not bear an interpretation in terms of native structure (Fersht, 1999)
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The development of oxidized flavor can be the result of several factors
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In contrast, the specifically-docked conformation (exemplified by the WT 4WJ) is stabilized by an extensive hydrogen bond network (14 hydrogen bonds in all (Rupert and Ferre-D'Amare, 2001)), prolonging the lifetime of this state
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