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For the longest sequence in our data set-the sequence of villin-for which N = 126, generation of the desired conformational ensemble takes ~ 20 h on a single 2 mane and tail shampoo .
The structure of EIN from Escherichia coli has been solved by x-ray (8) and NMR (9), and its complex with HPr has been described by NMR (10) mane and tail shampoo .
and thus, it was dominated by the emission of the sole tryptophan residue mane and tail shampoo .
In particular the wavelength of the tryptophan fluorescence emission peak (λ^sub max^) is a valuable parameter to characterize properties of the unfolded state mane and tail shampoo .
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Interestingly, the residues with (f = 0 mane and tail shampoo .
47 ml g^sup -1^, obtained from the R^sub h^ of the BLG monomer in dilute solution, leads to values of φ for the more concentrated solutions which are above the expected φ^sub max^ or, using the reverse of the above conversion, a mass fraction of 43 mane and tail shampoo .
DCVJ and ThT bound to aggregated TTR shows markedly similar fluorescence lifetimes which were overall short with at least three components (Fig mane and tail shampoo .
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4 D), on the other hand, a smooth continuation at 8 ns of the configurational entropy buildup is observed when the complex trajectory is appended to the free one (solid line), whereas a slight stepwise increase is visible when the trajectories are appended in the reverse order (dashed line) mane and tail shampoo .
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