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The column was then washed extensively with PBS till the washings had A^sub 280^Isothermal guanidinium-hydrochloride-induced denaturation Equilibrium unfolding as a function of guanidinium hydrochloride (GdnCl) concentration was monitored by fluorescence spectroscopy
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we used Metropolis Monte Carlo simulations to generate representative conformational ensembles in the EV limit
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We calculated absolute contact order using the method of Plaxco et al
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Changes in biomolecular conformation seen by small angle X-ray scattering
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To reduce the variability of fluorescence intensity in frozen samples, due to the poor homogeneity of the medium, the sample was rotated (3 Hz) during the measurement and the signal intensity was integrated for 10 s
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In an attempt to correlate affinities and n values to specific aspects of the macromolecule, we observe that all mutants are less stable than the WT by a comparable amount, the denaturation-free energy change, ΔG° at 20°C, decreasing from 6-7 to ~3 kcal/mole (20,27)
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The two cytoplasmic regions differed in their lipid contents and NMR lineshape characteristics-differences that are not detectable with whole-cell NMR techniques
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Lipid suppression has been especially important for selecting regions for localized spectroscopy, since at this high magnetic field strength, the lipid image of the lipid-rich oocyte significantly affects the water image, thus hampering correct voxel positioning
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Calcium/ calmodulin inhibition of transcriptional activity of E-proteins by prevention of their binding to DNA
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1 the osmotic pressure measurements indicate there are short-ranged attractive interactions at low ionic strength, but these result in liquid-liquid phase separation which is apparently unhindered by any kinetic arrest
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PROCEDURE FOR PRODUCING TONERThe effects of several polymer and process parameters on particle size were investigated (see Table III) and a suitable set of parameters of the required particle size was chosen
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Absolute fecundity estimates (based on fertilized eggs) ranged from 299,302 embryos for a 6-year-old female to 948,152 embryos for a 16-year-old female
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Steadman JR, Briggs KK, Rodrigo JJ
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The chain will bill the government directly for patients who purchase diabetic supplies, durable medical equipment or other health products covered by Part B, Alderson said
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Chapters discuss general poker concepts for the uninitiated, and the rules and favorable versus unfavorable situations for many poker variants including Limit Hold-em, Seven Card Stud, Eight Or Better Stud, Omaha High, Omaha Hi-Low, and No-Limit Hold-em
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There was a significant gender difference with males having a rate of probable pathological gambling double that of females (12
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The finding of a gender difference in the percentage of probable pathological gamblers is consistent with our previous results, but the failure to find a gender difference on the item of ever having gamble in a casino suggests that underage females are just as likely to enter a casino and gamble as under age males
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