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. Parameter A, which reflects the spectral shape of the intrinsic Trp fluorescence (31), was obtained by dividing the fluorescence intensity at 320 nm by the intensity at 365 nm
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. Throughout these discussions, advances in polymer theory that provide an appropriate framework for the description of denatured proteins have largely been ignored
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. 48 g/g) was measured by weighing shortly before closing the sample holder
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. To calculate the protein number density, the dimer molecular mass of 36
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. Our laboratory has previously characterized the affinity between PLCβs and their G-protein activators on model membrane surfaces using FRET (Runnels and Scarlata, 1999)
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. It should be noted, though, that entropies originating from different degrees of freedom need not be additive and that a decomposition will neglect correlation of motions along different degrees of freedom
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. Energetics of hydrogen bond networks in RNA: Hydrogen bonds surrounding G+1 and U42 are the major determinants for the tertiary structure stability of the hairpin ribozyme
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.These statements have not been evaluated by the Food and Drug Administration
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. The agency has only 40 people to review more than 30,000 promotions every year
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. The Mn^sup 2+^ and Ca^sup 2+^ concentrations used were 5 mM in all the experiments unless specified otherwise
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. Recently, Kohn et al
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. This erroneous model is of interest only because it has been used previously for denatured proteins in studies aimed at correlating m values and ΔC^sub P^ to changes in solventaccessible surface area (90)
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. Thermodynamics of protein folding: a microscopic view
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. interactions between EIN and EIC in the whole intact EI have been suggested (44)
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. We have developed a method for analyzing ^sup 15^N-spin relaxation measured at two magnetic fields that corrects (τ^sup B^^sub m^)^sub app^ for internal motion up to ~4 ns, giving (τ^sup o^^sub m^)^sub app-corr^ (22)
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. Furthermore, several residues in CaM also undergo conformational exchange on a timescale of ~50 µs
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. In comparison, the ThT fluorescence peak in a water-glycerol mixture remains constant at 492 nm
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. The melting data recorded at a scan rate 1
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. Such scan rate dependence, which was also observed in apoA-1:DMPC (Surewicz et al
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. In this case, the apparent crystallization temperature is less than the equilibrium melting temperature, and the monotectic point shifts slightly toward higher polymer concentrations as the cooling rate increases [10]
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. But it did so with 993 in-store pharmacies, three times the number wielded by its warehouse club competitor
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. Its pharmacies alone average more than $3
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.The company has said that the in-flight gambling system it has developed can be easily installed, requires no up-front costs and is supported by a comprehensive data tracking and financial management system to process the credit card transactions
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. College Student Journal 37, 60-62
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.A spokesperson for the company has stated that the observed increasecould have been due to the popularity of sporting events such as theWorld Cup and Wimbledon
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. The hands are quick and people understand the game because it's simple, even if you don't gamble," he said
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.Fear of busting (going over 21) was common
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