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14), also known as Acylase I (ACYI), is a homodimeric enzyme containing one zinc ion per subunit (4-6)
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Conversely, zooming out or scaling up from the contact density map of a short protein like PSBD will yield the contact density maps of longer proteins such as ubiquitin or titin
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These interactions will be determined by the specific sequence or, more precisely, by native-state topology
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Protein interactions and crystallization
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Brownsey,* Katrin Laos,[dagger] and Stephen G
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Aliquots of the aggregation reaction was withdrawn and assayed to avoid adverse effects of probe binding during the reaction
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7, we show the pressure dependence of CPM-Gβγ-PLCβ in buffer, which is presented as the fraction associated rather than the degree of dissociation to emphasize that these data are from changes in CPM-Gβγ fluorescence rather than in CPM-PLCβ
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Our studies of model discoidal (Gursky et al
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However, when a polymer-solvent system undergoes LLPS prior to crystallization in a certain range of polymer concentration (see Figure 1), the crystallization curve is modified
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Ratiometric single-molecule studies of freely diffusing biomolecules
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. Determination of act in content in actin preparions
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. For a given sequence, N residues long, we start with a random, sterically allowed conformation for the chain and calculate the inverse power potential energy U according to the formula shown in Eq
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. Contrary to interpretations of many such experiments, numerous molecular simulations (7,23,99) and recent single-molecule experiments (75) provide little evidence of long-range residual structure under harshly denaturing conditions
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. We calculated absolute contact order using the method of Plaxco et al
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. In all cases, the absolute value of the ellipticity decreased linearly, as the temperature was raised
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. Furthermore, trypsin digestion was reduced in the presence of PEP (either alone or with Mg^sup 2+^), but not in the presence of Mg^sup 2+^ alone
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. It may also be possible that a smaller degree of subunit dissociation in part explains the sharp increase in the residual enzymatic activity, retained after freeze-thawing, with increasing protein concentration (14,16,29)
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. 4, A and C) in the complex formed (dashed lines until 8 ns) toward the 8-ns time point are slightly higher (ΔS^sup 2nd,b-f^^sub gp120,bb^ = 0
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.68 *Note: * Statistically significant at Bonferroni adjusted valueof p < 0
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