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All other reagents were of the highest analytical grade available
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Refolding intermediate of guanidine hydrochloride denatured aminoacylase
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This work was supported by Projects CTQ2004-0771 ft from the Spanish Ministerio de Educación y Ciencia (MEC) to J
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Membrane binding for PLCβ was determined by following the increase in acceptor fluorescence from PLCβ Trp donors to model membranes doped with 0
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6 b) splitting over a certain distance, or, visible in Fig
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6), 10 mM MgCl^sub 2^, and 5 mM dithiothreitol for 30 min at 377deg;C
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Clearly, these simulations do not describe the data as well
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