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At the other end of the spectrum, recent experimental work and some modeling efforts suggest that a new paradigm is in order for denatured-state ensembles (100,101,130)
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In this work, we further investigate the stability and structure of El^sup sc^ at different pH in the absence and in the presence of its effectors (PEP and Mg^sup 2+^)
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DISCUSSION Structure of EI^sup sc^ in the absence of effectors We carried out studies at different pH using several spectroscopic probes to test whether the structure of EIC^sup sc^ changed
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Circular dichroism CD spectra in the far-UV region (200-280 nm) were recorded on a Jasco J-810 equipped with peltier thermostating cuvette holder under constant nitrogen flow
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Further, their internal flexibility increases drastically from compact WT and C3A/C26A to the cavity-forming mutants I7S and F110S
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Consequently, the k^sub ex^ values calculated in this way (Eqs
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Fluorescence accessory was used to record 90° light scattering simultaneously with the CD
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, 2003) on the actual dimensions of native insulin monomers (1
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RELATIONSHIP TO OTHER MODELSWe stress that the search-space picture explored above is not incompatible with the frameworks or results of the other models discussed in the introduction, but rather serves to show how the apparently alternative models are related, and how high-dimensional features are implicit in all of them
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The data in Table VI, along with T^sub m^, indicate that once T^sub m^ is exceeded, the IPP toner will flow much more readily than the EVA toners
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The hairpin ribozyme is a good model to explore this question because of the relatively simple secondary structure and the strong dependence of tertiary folding on the helical junction geometry
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Johnson said the move by Toyota has impacted his business negatively by about 15 percent
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