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Before the start of each experiment the temperature was calibrated with a water/DSS sample permanent make up rotary machine .
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The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG* permanent make up rotary machine .
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The observation of well-resolved subpopulations in the FRET efficiency histogram indicates that the ribozyme can adopt two distinct conformations in solution permanent make up rotary machine .
The FRET efficiency histogram of the GnI variant also exhibits two distinct subpopulations, with mean E values that are similar to the wild-type, but the amplitude of the high FRET peak is substantially reduced relative to the low FRET peak (Fig permanent make up rotary machine .
Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor permanent make up rotary machine .
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