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All the experiments were done in a Jobin Yvon Horiba fluorometer (Jobin Yvon Spex, Cedex, France) in a 1-cm water- jacketed cell using a protein concentration of 1 µM and ANS concentration of 50 µM
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Conversely, the contact density maps for the folded versions of different sequences reflect differences in native-state topologies
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Thermodynamic properties of the fluid and solid phases for inverse power potentials
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As for the biological aspects of this work, the noted high potential of Na+ cation in DNA compaction supports the notion that Na+ ion (contrary to K+) plays an important role in maintaining the compact higher order structure of DNA in vivo
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Such an effect would be quite improbable and surprising, should the fiber be produced by a spontaneous association of several free pre-existing protofilaments of the same length
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So the rapid, and dominant, search path coded in the interactions follows the order: helix (partial) formation-three-dimensional search (helix 1 and 3 out of contact)-two-dimensional search (helix 1 and 3 in contact)
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Yamada, J, and M
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RESULTSspFRET histograms reveal subpopulations of docked and extended ribozyme conformersFluorescence resonance energy transfer (FRET) is the nonradiative transfer of excitation energy between donor and acceptor dyes with overlapping emission and absorption spectra
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We thank Dagmar Klostermeier for assistance in preparing some of the hairpin ribozyme constructs
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--Pharmaceutical Sciences Social and Administrative SciencesSOURCE: AMERICAN ASSOCIATION OF COLLEGES OF PHARMACY <WWW
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