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Partial motivation for this reference line comes from the work of Zagrovic and Pande (78), who showed that internal distances in unfolded ensembles of several proteins follow the predictions of the ideal random-flight chain with link length of 3
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Cellular delivery of MRI contrast agents
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In soman-aged HuBChE, the organophosphoryl moiety remains covalently attached to the unfolded protein, and the salt-bridge between the protonated imidazole ring of His-438 and the negatively charged phosphoryl oxygens may contribute to the observed shift in the onset of unfolding to a higher temperature compared to the native enzyme
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At this pH (and pH 6
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We compared the properties of ThT with that of DCVJ and found a very similar dependence on viscosity after the increasing relative concentration of glycerol (Fig
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The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG*
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This suggests that two slightly different conformational spaces are sampled in the free and complex forms of the molecule, representing a situation of case A (Fig
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