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The enzymatic activity was determined by measuring the absorbance at 238 nm accompanied with hydrolysis of the substrate and using the molar absorption coefficient ε^sub 238^ = 185 M^sup -1^cm^sup -1^ as reported by Kordel and Schneider (5,6), except that chloroacetyl-L-leucine was used instead of pure-L-enantiomorph ronde barber picture .
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Denaturation of the native enzyme is reflected by a relative increase of MSDs consistent with entropic stabilization of the unfolded state ronde barber picture .
5%) was added, and the samples were boiled for 5 min before loading the SDS-PAGE gel ronde barber picture .
2, due to longer rotational correlation time of the aggregates than the fluorescence lifetime of ANS ronde barber picture .
X-ray crystal structure of lipid-free apoA-2 comprises a bundle of nearly parallel helices that opens upon lipid binding (Kumar et al ronde barber picture .
The second DSC peak is centered at 84°C, which is similar to the apparent T^sub m^ determined from the peak position in the first derivative of the CD melting data, dΘ^sub 222^(T)/dT (Fig ronde barber picture .
For example, a nonnative attraction beween the helices irrespective of their axial orientation would favor a two-dimensional search out of contact before a three-dimensional search in contact as the structure of the five-dimensional search space ronde barber picture .
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Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy ronde barber picture .
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