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. 1 A, solid squares); 3), the changes in solvent accessibility (Table 1); and 4), the absence of a sigmoidal thermal transition at low pH (Fig
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. The dynamic features presented here support the conclusion that the conformationally heterogeneous bHLH mimicking peptide trapped inside the CaM dimer exchanges between different binding sites on both nanosecond and microsecond timescales
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. In conclusion, residues with fast exchange can be identified from the combination of R^sub ex^ and ΔR^sub ex^ using either the fast exchange equations or the general fast/slow exchange equations derived by Ishima and Torchia (34)
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. The pressure behavior of membrane-bound CPM-PLCβ-Gβγ complexes were then studied in the presence of 50 nM Gα^sub s^(GDP) from atmospheric to 1000 kbar
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. Our results indicate that an early step in the 4WJ ribozyme folding pathway likely involves the formation of a fast equilibrium between extended undocked and quasi-docked structures (Fig
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. Structure and stability of El^sup sc^ in the presence of effectors The addition of the effectors to EI^sup sc^ did not alter substantially the secondary structure of the protein, as shown by FTIR (Table 4)
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. It was pointed out that this trend probably reflects multiple binding sites of varying affinity, likely a consequence of the broad distribution of protein conformations in ice (3)
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.Folding of single-loop ribozyme constructsThe results described in the preceding section suggest that Mg^sup 2+^-induced conformational changes within the helical junction region may control the approach of loops A and B during ribozyme folding
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