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Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown)
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Reversible dissociation and unfolding of dimeric creatine kinase isoenzyme MM in guanidine hydrochloride and urea
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Each measurement was an average of three readings
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the DSC scans show a single peak between pH 6
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Circular dichroismFar ultraviolet circular dichroism (UV CD) spectra were recorded using a Jobin Yvon (Longjumeau, France) circular dichroism spectropolarimeter in the range 190-260 nm
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3) identifies the various species populated at different time points
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11 B), amyloid fibrils of insulin, and lysozyme (P
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), The Foundation for Strategic Research (P
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Regulation of phosphoinositide-specific phospholipase C
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6, b and c, represents other morphological motifs commonly found in the insulin aggregate 10-min preparations at 70°C; for example, two laterally connected double-strand fibers of equal height of 3
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Manuscript approved for publication 16 September 2004 by the Scientific Editor
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The interrelation of the size offish eggs, the date of spawning and the production cycle
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major, indicating that egg samples preserved for up to 7 years could be analyzed by this method
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Ann Rheum Dis 2001;60:781-90
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