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Reversible dissociation and unfolding of dimeric creatine kinase isoenzyme MM in guanidine hydrochloride and urea
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As shown in Fig
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The rSBA clone that was in the pET 11b vector was transformed into Escherichia coli BL21 DE3 pLysS cells
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We speculate that under folding conditions, it is the typological distinction between the native and highly denatured states that provides part of the driving force for folding via collapse and symmetry breaking
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3 this behavior is shown for BAA
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The linewidths of the vegetal peaks were twice those of the animal peaks
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Atomic mean-square displacements (MSDs) were found to be identical for native HuBChE and for DmAChE in the whole temperature range examined, leading to the conclusion that differences in activity and substrate specificity are not reflected by a global modification of subnanosecond molecular dynamics
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An important time window in the picosecond-to-nanosecond time range is occupied by atomic thermal fluctuations, which have been extensively investigated by molecular dynamics (MD) simulations (2), and by various experimental approaches including neutron scattering (3,4) and nuclear magnetic resonance (NMR) (5)
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Second, the heat-unfolding curve of the apoA-2:DMPC disks is sigmoidal and is shifted to higher temperatures as compared to that of free protein; thus the unfolding of free apoA-2 is complete at 80°C, well below the apparent melting temperature T^sub m^ = 84°C of the apoA-2:DMPC complexes observed at this scan rate (Fig
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As development proceeded, larvae became slightly deeper and laterally compressed
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Then the eggs in the netwells were immersed in 100 mL of PBS in a polystyrene tray (Corning Incorporated, Corning, NY) for 5 minutes
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major (20 April, 1995) could react with the antibodies obtained as clearly as the recently collected eggs of P
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, 2000, 2002b), or to compact structures with partially formed subdomains of tertiary structure (Fang et al
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Academic stress of college students: Comparison of student and faculty perceptions
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