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4 µM) were incubated for 90 min in the absence or in the presence of different GdnHCl concentrations and applied onto a Superdex 200 HR column (Pharmacia, Uppsala, Sweden), previously equilibrated with 20 mM Tris-HCl, at pH 7
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Interactions of chaperone alpha-crystallin with the molten globule state of xylose reductase
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An estimation of the T^sub m^ of EIC^sup sc^, based on the fluorescence results (Fig
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Experiments conducted with copper-free azurin from Pseudomonas aeruginosa and mutants I7S, F110S, and C3A/C26A correlate the magnitude of the ice-induced perturbation, as inferred from the extent of ANS binding, to the plasticity of the globular fold, increasing with less stable globular folds as well as when the flexibility of the macromolecule is enhanced
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We first analyze the latter class and demonstrate that additional binding of ANS in ice is due to the creation of new binding sites rather than to a more complete saturation of preexisting weak sites after the large freeze-induced concentration of the ligand
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As a result, an oil-holding crosshatch with a required Ra is created, the company says
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Thus, (τ^sup o^^sub m^)^sub app^ contains information about the orientation of the N-H^sup N^ bond vector via the angle Φ
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3 ns, respectively, the anisotropy of the diffusion tensor is calculated to be 2
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A novel, specific interaction involving the Csk SH3 domain and its natural ligand
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Ultracentrifugal and electrophoretic studies of the association of β-lactoglobulin below its isoelectric point
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It is, however, not well reported that ANS also binds to aggregated protein molecules
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, 1999) and Alzheimer's β-amyloid peptides (LeVine, 1993)
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This lack of pressure stabilization may be due to the inability of the PH-PLCδ^sub 1^ to further penetrate the membrane surface to allow for stronger hydrogen bonding and ionic interactions
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The snout was longer and rounded, and relative head length was moderate
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Pacific Fisheries Management Council, Portland, OR,Wyllie Echeverria, T
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Quon and colleagues explained that there are fewer major manufacturers of generic drugs in Canada than in the U
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