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Secondary structure of proteins from circular dichroism spectra
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This is the case if at least one major component of the surrounding solvent is chemically equivalent to the main repeating unit of the polymer making chain-solvent contacts preferable to chain-chain contacts (4,37)
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MATERIALS AND METHODS Enzymes α-Amylase from Bacillus licheniformis (BLA; purchased from Sigma, Taufkirchen, Germany), from Bacillus amyloliquefaciens (BAA; purchased from Fluka, Buchs SG, Switzerland), from Bacillus subtilis (BSUA; from Fluka)
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Increased protein backbone conformational entropy upon hydrophobic ligand binding
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DCVJ was excited at 470 nm and ThT at 440 nm
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Fluorescent probes for polymer free-volume
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The plates were developed in chloroform/methanol/2
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Membrane binding of the G-protein subunits was followed by changes in the fluorescence of the CPM-labeled proteins (see Philip and Scarlata, 2004)
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, 2004b), lag phase is a typical feature of protein amyloidogenesis corresponding to the thermodynamically unfavorable nucleation process (Bouchard et al
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McLeish, University of Leeds, Physics and Astronomy, Woodhouse Lane, Leeds LS2 9JT, UK
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05, on ratings of the degree to which play outcome was due to their ability, nor was there an Advice/No-Advice X Block interaction, F [less than] 1
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